By Angela F. Drew
In Atherosclerosis: Experimental equipment and Protocols, Angela Drew and a panel of specialists have assembled a accomplished choice of conventional and state of the art ideas for investigating this ailment and its attainable remedies. each one quite simply reproducible strategy comprises step by step directions and sensible information about pattern assortment, the alternative of animal version process, experimental layout, and useful info research concepts. accomplished and richly special, Atherosclerosis: Experimental equipment and Protocols permits all biomedical investigators to pick these optimized thoughts which may be such a lot fruitfully used to check the advance, development, and therapy of atherosclerotic lesions this present day.
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Extra info for Atherosclerosis: Experimental Methods and Protocols (Methods in Molecular Medicine)
1997) Targeted replacement of the mouse apolipoprotein E gene with the common human APOE3 allele enhances diet-induced hypercholesterolemia and atherosclerosis. J. Biol. Chem. 272, 17972–17980. 16. Sullivan, P. , Quarfordt, S. , and Maeda, N. (1998) Type III hyperlipoproteinemia and spontaneous atherosclerosis in mice resulting from gene replacement of mouse Apoe with human APOE*2. J. Clin. Invest. 102, 130–135. 17. , Goldstein, J. , Brown, M. , and Burns, D. K. (1994) Massive xanthomatosis and atherosclerosis in cholesterol-fed low density lipoprotein receptor-negative mice.
9. All other chemicals or reagents not specified otherwise above are from Sigma Chemical Co. (St. Louis, MO), including cholesterol, triglyceride, and protein assay kits. Phospholipid assay kits are from Boehringer Mannheim (Germany). 04%). Adjust the solvent density (see Table 2) of the sample as determined by the equation above or by the addition of solid NaBr (see Table 3) to isolate the lipoproteins of interest. 006 g/ mL, VLDL and chylomicron particles are isolated. 21 g/mL, HDL particles are isolated.
2. Set up the fraction collector with the appropriate time or number of drops and collection vessel (see Notes 18,19,21). 3. If the Beckman unloader is used, connect the inlet at the top to the pump and the needle assembly at the bottom of the unloader to the fraction collector (see Note 8; Fig. 1C). 4. Start the pump. When the tube joining the unloader to the fraction collector is filled with the displaced gradient, begin the collection of fractions. 4. 1. Lipid Analysis (see Notes 10 and 11) 1.
Atherosclerosis: Experimental Methods and Protocols (Methods in Molecular Medicine) by Angela F. Drew