By Lyn Healy, Ludmila Ruban
This lavishly-illustrated, authoritative atlas explores the complex paintings of culturing human pluripotent stem cells. Twelve chapters – containing greater than 280 colour illustrations – disguise numerous issues in pluripotent stem cellphone culturing together with mouse and human fibroblasts, human embryonic stem cells and caused pluripotent stem cells, attribute staining styles, and irregular cultures, between others. Atlas of Human Pluripotent Stem Cells in tradition is a entire choice of illustrated ideas complemented by means of informative and academic captions interpreting what quality cells seem like and the way they behave in numerous environments. Examples of excellent cultures are in comparison side-by-side to less-than-perfect and unacceptable examples of human embryonic and precipitated pluripotent stem phone colonies. This targeted and thorough atlas is a useful source for researchers, lecturers, and scholars who're attracted to or operating with stem telephone culturing.
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Additional info for Atlas of Human Pluripotent Stem Cells in Culture
C) Another good colony similar to the one in a (all ×10 magnification) 4 Human Embryonic Stem Cells Fig. 15 There is spontaneous differentiation (white arrows) on the edges of this colony, but the rest of the colony is undifferentiated (×4 magnification) a Fig. 17 (a) The large colony on the right side is of a relatively good morphology. The smaller colony on the left is not compacted, does not have smooth edges, and the cells are disorganised. Because of these features, the colony is probably going to differentiate within a day or 43 Fig.
However, in co-culture with appropriate media and media changes, the PSCs can remain undifferentiated for up to 2 weeks. As feeder layers age, the feeder cells start to deteriorate and lose their ability to prevent the differentiation of PSCs. Feeder cells maintained in media formulations optimised for use in co-culture with PSCs show a change in morphology over time, acquiring an elongated, spindle-shaped phenotype indicative of stress. 33 Feeder cells are therefore maintained in the appropriate media until used in co-culture with PSCs (Fig.
The dissociated cells should be round, single and refractile (bright ring around the outside of the cell) (Both ×10 magnification) a Fig. 5 (a, b) Comparison between two different densities of human fibroblast feeders (derived from human dermal fibroblasts), 24 h after passaging. Both cultures are of good quality. As with their murine b counterparts, the cells are uniform and evenly spread, with few apoptotic cells and little floating debris (Both ×4 magnification) 22 a Fig. 6 Human feeders at Day 2 after passaging (a) and Day 4 (b).
Atlas of Human Pluripotent Stem Cells in Culture by Lyn Healy, Ludmila Ruban