By Raymond J. Shamberger (auth.)
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Extra info for Biochemistry of Selenium
McCready, R. G. , Campbell, J. , and Payne, J. , 1966. Selenite reduction by Salmonella heidelberg, Can. J. Microbiol. 12:703-714. Mills, G. , and Randall, H. , 1958. Hemoglobin catabolism. II. The protection of hemoglobin from oxidative breakdown in the intact erythrocyte, J. Bioi. Chem. 232:589-598. , 1974. Purification and properties of rat liver glutathione peroxidase, Biochim. Biophys. Acta 358:251-261. Nigam, S. , and McConnell, W. , 1972. Isolation and identification of L-cystathionine and L-selenocystathionine from the foliage of Astragalus pectinatus, Phytochemistry 11 :377-380.
Res. Commun. 31:145-150. Ljungdahl, L. , and Andreesen, J. , 1975. Tungsten, A component of active formate dehydrogenase of Clostridium thermoaceticum, FEBS Lett. 54:279-282. , 1959. The microestimation of succinate and the extinction coefficient of cytochrome C, Biochim. Biophys. Acta. 34:255-256. McCollach, R. , Hamilton, J. , and Brown, S. , 1963. An apparent seleniferous leaf wax from stanleya bipinnata. Biochem. Biophys. Res. Commun. 11:7-13. McConnell, K. , and Portman, O. , 1952. Excretion of dimethyl selenide by the rat, J.
In addition, all other amino acids normally found in proteins were also present. Mter digestion of the protein with nitric acid and perchloric acid, the selenium content of the enzyme was measured by fluorimetric analysis. Nakamura et al. (1974) found 4 gatoms/mol, which is in agreement with the results of other investigations. If the enzyme was dialyzed for two days against five changes of 50 mM sodium phosphate buffer, pH 7 containing p-chloromercuribenzoate, titration showed the presence of 8 equiv of SH per mole of protein, which accounts for the two cysteine residues per subunit.
Biochemistry of Selenium by Raymond J. Shamberger (auth.)